Issue: Vojnosanit Pregl 2017; Vol. 74 (No. 12)
Association between Val158Met COMT, TNF-α -857 C>T, TNFR1 36 A>G, IL-1α 4845 G>T and IL-10 -1082 A>G polymorphisms and risk of early-onset preeclampsia and its complications
Authors:
Tijana Krnjeta, Ljiljana Mirković, Svetlana Ignjatović, Dragana Tomašević, Jelena Lukić, Drina Topalov, Nada Majkić-Singh
Background/Aim. Preeclampsia (PE) belongs to the group ofhypertensive disorders in pregnancy with the global average incidence
of 2.16%. It is considered as one of the leading causes of
maternal and neonatal morbidity and mortality worldwide. The
goal of this study was to assess the potential association between
Val158Met catechol-o-methyltransferase (COMT), tumor necrosis
factor-alpha (TNF-α) -857 C>T, tumor necrosis factor receptor
1 (TNFR1) 36 A>G, interleukin-1alpha (IL-1α) 4845 G>T
and interleukin-10 (IL-10) -1082 A>G polymorphisms and risk
of early-onset preeclampsia (PE) and its complications. Methods.
The study included 47 early-onset PE patients, which were
grouped by disease severity and by size for gestational age and 47
control cases. The Val158Met polymorphism was genotyped by
polymerase chain reaction – restriction fragment length polymorphism
(PCR-RFLP) analysis and inflammatory cytokine
polymorphisms by the Sanger sequencing method. Results. The
COMT Met allele as well as IL-1α T showed a protective role,
decreasing the risk of early-onset PE after age and body mass index
(BMI) adjustments. The detected interactions between the
COMT Met and IL-10 A alleles, as well as between the COMT
Met and TNF-α T alleles were insignificant after age and BMI
adjustments. Conclusion. COMT and IL-1α may be used as
candidate genes for early-onset PE and its severe form and small
for gestational age (SGA) complications
