Issue: Vojnosanit Pregl 2017; Vol. 74 (No. 12)
Relationship between IL-1β production and endodontic status of human periapical lesions
Authors:
Lidija Popovska, Cena Dimova, Biljana Evrosimoska, Vera Stojanovska, Ilijana Muratovska, Bojana Cetenović, Dejan Marković
Background/Aim. Apical periodontitis is mainly caused by bacterialinfection within the root canal and periapical bone destruction
which are prominent features of this lesion. The aim of this study
was to determine the quantity of interleukin-1β in the tissues of
periapical lesions and to analyze its relationships with: lesion size,
previous treatments and pathohistological finding of involved teeth.
Methods. Periapical tissues were obtained from patients undergoing
periapical surgery. Out of all 80 cases included in the study, 24
had no previous endodontic treatment (open lesions), 37 were with
endodontic failure (closed lesion) and in 15 cases root canal retreatment
was performed few months before the surgery. By excluding
four samples, the total of 76 samples, consisted of periapical
lesions and the apical part of the tooth root, was collected. Each
periapical tissue sample was divided into two equal parts. The one
half of each lesion was used for quantification of interleukin-1β in
tissue homogenates by the enzyme-linked immunosorbent assay
(ELISA) method. The other part of each lesion was used for histopathological
evaluation. Results. For each of the tissue homogenates,
the quantity of interleukin-1β was measured, and it ranged
from 0.6 pg/mg up to 74 pg/mg. There was no significant difference
between the symptomatology and amount of interleukin-1β.
Statistical data analysis showed a moderate correlation between lesion
size and interleukin-1β measured values. The highest levels of
interleukin-1β corresponded with chronic lesions in the stages of
acute exacerbation and granulomas in early developing stages. Persistant
granulomas, scar tissues, non-inflamed cysts and teeth with
recently finished endodontic treatments showed a significantly
lower level of interleukin-1β. Conclusion. The study results suggest
that the differences in quantity of interleukin-1β correlate to lesion
progression and phases of development.
