Background/Aim. Tinnitus is a prevalent auditory condition that significantly affects the quality of life. Despite the variety of treatments available, their effectiveness is inconsistent. The aim of this study was to evaluate the impact of a newly developed sound therapy system on tinnitus patients, specifically in terms of changes in audiology, tinnitus-related tests, and tinnitus psychoacoustic characteristics. Methods. A total of 100 patients with tinnitus were included in the study. They were divided into two groups: the control group (CG) (n = 48) and the treatment group (TG) (n = 52). Various demographic characteristics of the patients were recorded. All patients had a thorough audiological assessment, which included pure tone audiometry and acoustic immittance testing, as well as tinnitus-specific evaluations, such as tinnitus frequency matching, tinnitus loudness matching, minimum masked level (MML), and residual inhibition (RI) examinations. In addition, psychoacoustic characteristics of tinnitus were assessed, including Tinnitus Handicap Inventory (THI), Hospital Anxiety and Depression Scale (HADS), Pittsburgh Sleep Quality Index (PSQI), and Visual Analog Scale (VAS) scores. Results. There were no significant differences in various demographic characteristics between CG and TG before acoustic therapy. Additionally, there were no significant differences between the groups in audiometric assessments, tinnitus-related tests, psychological acoustic characteristics before treatment, or in the distribution of tinnitus frequencies. The hearing threshold level in TG was notably lower than in CG (p = 0.0238). Tinnitus loudness was significantly reduced in TG (p = 0.0256). MML in TG tended to improve (p = 0.0532), although this difference did not reach statistical significance and showed a positive trend. Among the subscales of THI, TG demonstrated significant improvements. PSQI scores also indicated they were significantly lower in TG than in CG (p = 0.0238). The total score on HADS (16.58 ± 2.89 vs. 16.67 ± 2.76; p = 0.8730) and the total score on VAS (4.19 ± 1.48 vs. 4.22 ± 1.37; p = 0.9160) did not show a significant difference. Conclusion. Acoustic therapy significantly improves patients’ hearing, reduces tinnitus loudness, and improves sleep quality. Our acoustic therapy system is an effective strategy for alleviating chronic subjective tinnitus.